I have followed three steps.
1. Scanned PC with Pendrive with AVG Antivirus
2. Scanned with Microsoft Security Essentials after downloading from Microsoft downloading centre.
3.Followed the steps--
* Click on "Start" -->Run-->type cmd and click on OK.
* Here I assume your pendrive as G:
* Enter this command.
* attrib -h -r -s /s /d g:\*.*
* Note : Replace the letter g with your flash drive letter.
4. Deleted the Shortcuts after verifying the mian folders.